Program
| MSB1a | |
|---|---|
| Raibatak Das | |
| University of British Columbia | |
| Title | Imaging and modeling the recruitment of cytosolic signaling proteins to the plasma membrane |
| Abstract | The recruitment of cytosolic signaling proteins to the plasma membrane is a key step in a variety of intracellular signaling cascades initiated by the activation of membrane-associated receptors. The translocation of a cytosolic protein is frequently observed within seconds to minutes following receptor activation. The in vivo dynamics of this process are typically difficult to quantify accurately. As a prototypical example for such a system, we study the recruitment of the adaptor protein Gab-1 to the plasma membrane of B lymphocytes upon B cell receptor crosslinking. We use a combination of confocal fluorescence microscopy to observe this process in individual cells under physiological conditions, and image processing tools to quantify the recruitment dynamics. In this talk I will present the details of our methodology and the efforts to model and parametrize this phenomenon. |
| Location | Woodward 1 |