| CTG7a | |
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| Julia Arciero |
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| University of Pittsburgh |
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| Title | Predicting migration of the intestinal epithelial cell layer using a continuum mechanical model |
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| Abstract | The rapid migration of intestinal epithelial cells at a wound edge is an important initial step in tissue repair. Mathematical modeling of this process may provide insight into the mechanisms that govern cell migration under normal and disease conditions. Here, a two-dimensional continuum mechanical model is used to simulate the motion of the epithelial layer in response to a wound. The model incorporates the effects of the forces acting on the wound edge and induced by lamellipod formation, the adhesion forces between the cell layer and the substrate, and the elastic stress of the cell layer due to the deformation. The model is solved numerically using a level set method, which tracks the moving boundary on a fixed grid. Initial coordinates for the simulated wound are defined using data from experimental cell migration images. Time to wound closure is shown to depend on initial wound shape. The location and velocity of the wound edge predicted by the model is compared with the position and velocity of the recorded wound edge. These comparisons show good qualitative agreement between model results and experimental observations. |
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| Coauthors | Qi Mi, Kristen Pueschel, Maria Branca, David Hackam, David Swigon |
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| Location | CHBE 102 |
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