| Abstract | Observing bacterial FtsZ GTP dependent polymerization in vitro is challenging experimentally given the dynamic nature of the process and the small size of the individual filaments. We present results obtained from observing the depolymerization process of individual filaments formed under GTP hydrolyzing conditions with atomic force microscopy. Observation of the structures formed at different protein densities and under different nucleotide hydrolysing conditions indicates the curved, dynamic and flexible character of the filaments and provides insight into the way filaments assemble and disassemble. |
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