| Poster PS16B | |
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| Jennifer Morrison |
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| UBC |
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| Title | Quantifying transient directed motion in single particle tracks using a hidden Markov model |
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| Abstract | Single Particle Tracking (SPT) is a widely used biophysical technique whereby a biomembrane component is fluorescently or optically tagged and its trajectory is observed. Analysis of SPT data has led to investigations of the different modes of particle motion, the binding kinetics of specific proteins and the underlying structure of the plasma membrane. Quantification of a protein’s interactions with the cytoskeleton and identification of directed motion provides insight into the spatiotemporal organization of the signaling pathways involved. I will present a method to identify transient directed motion in tracks of membrane-bound proteins using a hidden Markov model. As shown with simulated data, we can accurately estimate the diffusion coefficients and directed velocities of a particle in each state as well as the transition rates between each state. I will also discuss our attempts at analyzing particle tracks on T Cells using this method. |
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| Coauthors | Daniel Coombs, Raibatak Das |
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| Location | Woodward Lobby (Wednesday-Thursday) |
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