| Abstract | Budding yeast, Saccharomyces cerevisiae, respond to temperature changes by activating or repressing the transcription of genes. While the response to heat has been well studied, little is known about which transcription factors regulate the response to cold. Bioinformatic analysis suggested that Cin5p was a candidate regulator, so a cold shock experiment was performed on the yeast deletion strain BY4741Δcin5. Early log phase cells grown at 30°C were shifted to 13°C for one hour and then recovered at 30°C for an additional hour. Cells were harvested at six time points, and the expression ratios of the genes compared to the initial time point at 30°C were measured using DNA Microarrays. Wild type microarray data from an identical cold shock experiment were compared to the Δcin5 dataset. Although most gene expression changes observed in the Δcin5 strain were similar to those seen in wild type yeast, 16% of the known Cin5p target genes had different expression profiles in the two strains. Cin5p target genes showing expression changes in the deletion strain suggests cross-talk by other related transcription factors or by compensation by the other members of the YAP-family. The Δcin5 strain was not impaired for growth at cold temperatures either on sold media or in liquid culture. However the Cin5p over-expression strain was impaired at cold, suggesting that Cin5p’s role in regulating the response to cold is more complex than originally hypothesized. |
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